Table 1.
S. cerevisiae strains used in this study
| Straina | Genotype | Reference or source |
|---|---|---|
| ABY530 | MATα lys2-801 ura3-52 his3Δ-200 trp1Δ-63 leu2-3,112 ade2-101 myo2-20::HIS3 | Schott et al. (1999) |
| ABY944 | MATalys2-801 ura3-52 his3Δ-200 trp1-1 leu2-3,112 tpm1-2::LEU2 tpm2Δ::HIS3 | Pruyne et al. (1998) |
| K1993 | MATaura3 his3-11,15 trp1-1 leu2-3,112 ade2-1 can1-100 ssd1-Δ2 cdc15-2 | A gift from K. Nasmyth |
| YMW221U | MATalys2-801 ura3-52 his3Δ-200 trp1Δ-63 leu2Δ-1 ade2-101 arp2-2(G19D)::URA3 | Madania et al. (1999) |
| YEF473 | MATa/α lys2-801/lys2-801 ura3-52/ura3-52 his3Δ-200/his3Δ-200 trp1Δ-63/trp1Δ-63 leu2Δ-1/leu2Δ-1 | Bi and Pringle (1996) |
| YEF2182 | MATabni1Δ::HIS3 bnr1Δ::HIS3 [YEp352-BNI1] | This study |
| YEF2632 | MATabni1Δ::TRP1 bnr1Δ::HIS3 [pRS316-BNR1] | This study |
| YEF2669 | MATabni1-116 bnr1Δ::HIS3 | This study |
| YKT38 | MATalys2-801 ura3-52 his3Δ-200 trp1Δ-63 leu2Δ-1 | Mochida et al. (2002) |
| YKT91 | MATamyo3Δ::TRP1 myo5-1::KanMX6 | Toi et al. (2003) |
| YKT382 | MATabni1Δ::HIS3MX6 | This study |
| YKT440 | MATabnr1Δ::HphMX4 HIS3::PGAL1-3HA-BNI1 | This study |
| YKT483 | MATacla4Δ::KanMX6 HIS3::PGAL1-3HA-BNI1 | This study |
| YKT541 | MATabni1Δ::HIS3MX6 cla4Δ::KanMX6 URA3::cla4-75-td | This study |
| YKT618 | MATacla4Δ::KanMX6 URA3::cla4-75-td | This study |
| YKT637 | MATaLEU2::CDC12-EGFP | This study |
| YKT638 | MATabni1-116 cla4Δ::KanMX6 LEU2::CDC12-EGFP | This study |
| YKT649 | MATabni1Δ::HIS3MX6 cla4Δ::KanMX6 URA3::cla4-75-td LEU2::CDC12-EGFP | This study |
| YKT664 | MATabud6Δ::HphMX4 cla4Δ::KanMX6 URA3::cla4-75-td LEU2::CDC12-EGFP | This study |
| YKT666 | MATaspa2Δ::HphMX4 cla4Δ::KanMX6 URA3::cla4-75-td LEU2::CDC12-EGFP | This study |
| YKT668 | MATapea2Δ::HphMX4 cla4Δ::KanMX6 URA3::cla4-75-td LEU2::CDC12-EGFP | This study |
| YKT676 | MATabni1Δ::HphMX4 LEU2::CDC12-EGFP | This study |
| YKT678 | MATacla4Δ::KanMX6 URA3::cla4-75-td LEU2::CDC12-EGFP | This study |
| YKT717 | MATacla4Δ::KanMX6 LEU2::CDC12-EGFP | This study |
| YKT743 | MATabni1-116 bnr1Δ::HphMX4 LEU2::CDC12-EGFP | This study |
| YKT744 | MATabni1-116 bnr1Δ::HphMX4 cla4Δ::KanMX6 LEU2::CDC12-EGFP | This study |
| YKT811 | MATabni1Δ::HIS3MX6 cla4Δ::KanMX6 TRP1::cla4-75-td LEU2::CDC12-EGFP | This study |
| YKT812 | MATatpm1-2::LEU2 tpm2Δ::HIS3 LEU2::CDC12-EGFP | This study |
| YKT813 | MATatpm1-2::LEU2 tpm2Δ::HIS3 cla4Δ::KanMX6 LEU2::CDC12-EGFP | This study |
| YKT814 | MATamyo2-20::HIS3 LEU2::CDC12-EGFP | This study |
| YKT815 | MATamyo2-20::HIS3 cla4Δ::KanMX6 LEU2::CDC12-EGFP | This study |
| YKT816 | MATaarp2-2(G19D)::URA3 LEU2::CDC12-EGFP | This study |
| YKT817 | MATaarp2-2(G19D)::URA3 cla4Δ::KanMX6 LEU2::CDC12-EGFP | This study |
| YKT818 | MATamyo3Δ::TRP1 myo5-1::KanMX6 LEU2::CDC12-EGFP | This study |
| YKT819 | MATamyo3Δ::TRP1 myo5-1::KanMX6 cla4Δ::CgHIS3 LEU2::CDC12-EGFP | This study |
| YKT891 | MATaLEU2::CDC12-EGFP URA3::PGAL1-GFP-CDC42 | This study |
| YKT892 | MATaLEU2::CDC12-EGFP URA3::PGAL1-GFP-CDC42G12V | This study |
| YKT893 | MATabni1Δ::HphMX4 LEU2::CDC12-EGFP URA3::PGAL1-GFP-CDC42 | This study |
| YKT894 | MATabni1Δ::HphMX4 LEU2::CDC12-EGFP URA3::PGAL1-GFP-CDC42G12V | This study |
| YKT939b | MATacdc15-2 LEU2::CDC12-EGFP | This study |
| YKT940b | MATabni1Δ::KanMX6 cdc15-2 LEU2::CDC12-EGFP | This study |
a
YEF and YKT strains, except for YKT939 and YKT940, are isogenic derivatives of YEF473. For YEF and YKT strains, only relevant genotypes are described.
b
These strains are derivatives of K1993.