Table 2.
Plasmids used in this study
| Plasmid | Characteristics | Reference or source |
|---|---|---|
| pFA6a-TRP1 | TRP1 | Longtine et al. (1998b) |
| pFA6a-KanMX6 | PTEF-KanMX6-TTEF | Longtine et al. (1998b) |
| pAG32 | PTEF-HphMX4-TTEF | Goldstein and McCusker (1999) |
| pcla4-75-degron | cla4-75-ts degron URA3 | Weiss et al. (2000) |
| YCp50-LEU2-BNI1 | BNI1 LEU2 CEN4 | A gift from J. Pringle |
| YEp352-BNI1 | BNI1 URA3 2 μm | A gift from J. Pringle |
| YEp24 | URA3 2 μm | Rose and Broach (1990) |
| pRS316 | URA3 CEN6 | Sikorski and Hieter (1989) |
| pRS316-BNR1 | BNR1 URA3 CEN6 | This study |
| pKT1178 [pRS316-CDC12G] | CDC12-EGFP URA3 CEN6 | Iwase and Toh-e (2001) |
| pKT1254 [pRS316-CLA4] | CLA4 URA3 CEN6 | This study |
| pKT1364 [YEp24-BEM3(Δ1-114)] | BEM3 (Δ1-114) URA3 2 μm | This study |
| YEplac195 | URA3 2 μm | Gietz and Sugino (1988) |
| pKT1365 [YEplac195-RGA1(Δ1-632)] | RGA1 (Δ1-632) URA3 2 μm | This study |
| pRS314 | TRP1 CEN6 | Sikorski and Hieter (1989) |
| pKT1226 [pRS314-BNI1] | BNI1 TRP1 CEN6 | This study |
| pKT1420 [pRS314-BNI1(Δ6-808)] | BNI1 (Δ6-808) TRP1 CEN6 | This study |
| pKT1421 [pRS314-BNI1(Δ826-986)] | BNI1 (Δ826-986) TRP1 CEN6 | This study |
| pKT1422 [pRS314-BNI1(1-1750)] | BNI1 (1-1750) TRP1 CEN6 | This study |
| pKT1423 [pRS314-BNI1(Δ1228-1414)] | BNI1 (Δ1228-1414) TRP1 CEN6 | This study |
| pKT1424 [pRS314-BNI1(Δ1553-1646)] | BNI1 (Δ1553-1646) TRP1 CEN6 | This study |
| pRS305 | LEU2 | Sikorski and Hieter (1989) |
| pKT1452 [pRS305-CDC12-EGFP] | CDC12-EGFP LEU2 | This study |
| pRS306 | URA3 | Sikorski and Hieter (1989) |
| pKT1474 [pRS306-PGAL1-GFP-CDC42] | PGAL1-GFP-CDC42 URA3 | This study |
| pKT1475 [pRS306-PGAL1-GFP-CDC42G12V] | PGAL1-GFP-CDC42G12V URA3 | This study |