Table 1.

Primer sequences

Plasmid Construction
Construct	Primer Sequences	Restriction sites	Vector
pSRα-HA-TIA-1	5′-CACAGAATTCATGGAGGACGAGATG-3′	EcoRI	pSRα-HA
	5′-TATATAGTCGACTCACTGGGTTTCATAC-3′	SalI
pSRα-HA-RRM	5′-CACAGAATTCATGGAGGACGAGATG-3′	EcoRI	pSRα-HA
	5′-TATATAGTCGACTCATAGTTGTTCTGTTAGC-3′	SalI
pSRα-HA-PRD	5′-CACAGAATTCATGCGTCAGACTTTTTC-3′	EcoRI	pSRα-HA
	5′-TATATAGTCGACTCACTGGGTTTCATAC-3′	SalI
pSRα-HA-Sup35NM	5′-CACAGAATTCATGTCGGATTCAAACC-3′	EcoRI	pSRα-HA
	5′-TATATAGTCGACTCACAAATTGTTATTGTAGTTG-3′	SalI
pSRα-HA-RRM/NM	5′-CACAGAATTCATGGAGGACGAGATG-3′	EcoRI	pSRα-HA
	5′-TATATCTAGATAGTTGTTCTGTTAGC-3′	XbaI
	5′-TATATCTAGAATGTCGGATTCAAACC-3′	XbaI
	5′-TATATAGTCGACTCACAAATTGTTATTGTAGTTG-3′	blunt
pcDNA3-FLAG-HSP70	5′-CTCTCGGATCCGCCAAGAACACGGCGATC-3′	BamHI	pcDNA3-FLAG
	5′-CACACGAATTCCTAATCCACCTCCTCGAT-3′	EcoRI
pcDNA3-FLAG-HSC70	5′-CTCTCGGATCCAAGGGACCTGCAGTTGGC-3′	BamHI	pcDNA3-FLAG
	5′-CACACGAATTCTTAATCCACCTCTTCAAT-3′	EcoRI
Digoxygenin-labeled DNA probes
Probe	Primer sequence	Fragment size	Template
HSP27	5′-GTCAAGACCAAGGATG-3′	229 bases	pcDNA3-HSP27
	5′-GACTCGAAGGTGACTG-3′
HSP70 gene 2 UTR	5′-AAGTGGACTGTTGGGACTCAAGGACTTTG-3′	239 bases	Heat shocked
	5′-CAAACAAACTCGTACAGAAGGTG-3′		COS7 cDNA
RT-PCR analysis
	Primer sequence
HSP70 coding region	5′-ATAACGGCTAGCCTGAGGA-3′
	5′-GTCCGACTGCACCACCGGG-3′
HSP70 gene 1 UTR	5′-GAGCTTCAAGACTTTGCATTTCTTAG-3′
	5′-GGGCATCACTTGAATTTTAAAG-3′
HSP70 gene 2 UTR	5′-AAGTGGACTGTTGGGACTCAAGGACTTTG-3′
	5′-CAAACAAACTCGTACAGAAGGTG-3′
ATF4	5′-CTGTGGGTCTGCCCGTCCCAAAC-3′
	5′-TCAACTAGGGGACCCTTTTC-3′
GADD34	5′-GAGCAGCTTGCTCGGGATCGC-3′
	5′-TCAGCCACGCCTCCCACTG-3′
XBP1	5′-CTTTGTGGTTGAGAACCAGG-3′
	5′-GGGAGCTCCTCCAGGCTG-3′