Figure 2.

Immunolocalization of the GFP-α₁ subunit constructs in calcium release units of reconstituted dysgenic myotubes. GLT myotubes were transfected with a range of GFP-tagged α₁ subunit isoforms and chimeras. Double immunofluorescence labeling with anti-GFP (left column) and anti-RyR1 (middle column) shows that all α₁ subunits and chimeras are located in clusters that are colocalized with RyR1 clusters (examples indicated by arrows). Colocalization of α₁ subunit clusters and RyR1 clusters is indicative of their localization in T-tubule/SR and plasma membrane/SR junctions. In the merged color image (right column) colocalization of anti-GFP stain (green) and anti-RyR1 stain (red) results in yellow clusters. Bar, 10 μm.