Figure 2.

Late endosome morphology is altered in Rab9 depleted cells. (A) Immunofluorescence microscopy of HeLa cells transfected with control siRNA (left) or Rab9 siRNA (right) and double labeled with monoclonal anti-Rab9 antibody (top) or polyclonal anti-Rab7 antibody (bottom). (B) Control-transfected (left) or Rab9 siRNA-transfected (right) HeLa cells stained with monoclonal anti-LAMP1 antibody. The bottom panel pair is shown at higher magnification and outlined to highlight the distinction between control and depleted cells.