Figure 1.

Inner arm dynein I1 and IC138 cloning strategy. (A) Illustration of the inner arm dynein I1. The I1 dynein complex is composed of two heavy chains (1α Dhc and 1β Dhc), three intermediate chains (IC140, IC138, and IC97—also called IC110), and several light chains (LC8, LC7a, LC7b, Tctex1, and Tctex2b). (B) Primers (P5S and P1AS) were designed from sequence obtained from purified IC138 and used for RT-PCR. Sequences obtained by RT-PCR were then used to design additional primers for screening Chlamydomonas genomic libraries. The sequences obtained from those screens were then used to screen cDNA libraries to generate the full-length IC138 cDNA. The IC138 gene contains 11 exons (solid bars). The IC138 sequence can be obtained from GenBank under the accession no. AY743342.