Figure 2.

The IC138 gene encodes a WD-repeat containing protein. The IC138 cDNA predicts a protein of 1057 amino acids and ∼111 kDa with a calculated pI of 5.73. Peptide sequences obtained from microsequencing purified IC138 are underlined; the N-terminal sequence that was used to create the His-tagged fusion protein for the purpose of producing the IC138 polyclonal antibody is italicized. IC138, like other IC dyneins, is predicted to contain seven WD-repeats, which are indicated in bold. The residues enclosed in the shaded box are missing in bop5-1.