Figure 5.

IC138 rescues motility in bop5-1. bop5-1 cells that had been transformed with full-length IC138 and stable diploids containing both the wild-type IC138 gene and the bop5-1 IC138 gene were analyzed. (A) Swimming speeds of freely swimming cells were measured. The decreased swimming speed seen in bop5-1 is rescued by wild-type IC138. (B) By Western analysis, only the full-length IC138 is found in the axonemes of the stable diploids (#1, #2, and #4) and the bop5-1 cells transformed with the wild-type IC138 gene (2A, E6).