Figure 6.

LC7b fails to assemble in I1 from bop5-1. (A) Western analyses were performed on axonemes isolated from wild-type and bop5-1 cells by using antibodies to the 1α dynein heavy chain (1α Dhc), two intermediate chains (IC138 and IC140), and the light chains (LC8, Tctex1, and Tctex2b). (B) Silver-stained SDS-PAGE of I1 fractions indicating that IC97 (arrowhead) is assembled in axonemes from bop5-1 cells in an amount comparable with the wild-type positive control derived from pf28 axonemes and lacking in the negative control derived from pf28pf30 axonemes. IC140, IC138, and truncated IC138 are indicated with asterisks. (C) Further analysis of bop5-1 and the double mutant bop5-1oda7 reveals that LC7b, but not LC7a fails to assemble when IC138 is truncated. The tubulin region of the gel was excised and Coomassie stained to demonstrate protein loads.