Figure 8.

IC138 is hyperphosphorylated in radial spoke and central pair mutants. To determine whether of the phosphorylation state of IC138 is altered in paralyzed flagellar mutants, axonemes isolated from wild-type, pf17, pf18, mia2-1, bop5-1, and bop5-1pf17 were treated with CIP. (A) Based on a comparison of CIP-treated (-) versus control, untreated (+) axonemes, IC138 seems to be hyperphosphorylated in mia2-1 and the paralyzed mutants pf17, pf18, and bop5-1pf17. In contrast, IC138 from wild type and bop5-1 is not hyperphosphorylated. (B) Flagella were isolated and demembranated immediately preceding the addition of ATP and protease. Microtubule sliding velocity is expressed as micrometers per second.