Table 1.
S. cerevisiae strains used in this study
| Strain | Background | Genotype | Source |
|---|---|---|---|
| yEF569HA | A364a | MATaleu2 trp1 ura3 his3 mec1::TRP1 sml1-1 RAD9-3×HA pipo | Our laboratory |
| yEF569HA-81 | A364a | MATaleu2 trp1 ura3 his3 mec1::TRP1 sml1-1 RAD9-3×HA pipo Δddc2::KANR | Our laboratory |
| U960-5C | W303 | MATaade2-1 can1-100 his 3-11,15 leu2-3, 112 trp1-1 ura 3-1 sml1-1 rad53-XB::HIS3 | R. Rothstein |
| ySJL101-1 | W303 | MATaade2-1 can1-100 his 3-11,15 leu2-3, 112 trp1-1 ura 3-1 sml1-1 rad53-XB::HIS3 DDC2 FLAG::LEU::ddc2 | Our laboratory |
| yJKD 417 | W303 | MATaade2-1 can1-100 his 3-11,15 leu2-3, 112 trp1-1 ura3 sml1-1 rad53-XB::HIS3 Δmec1::TRP | Our laboratory |
| ySJL104-1 | W303 | MATa ade2-1 can1-100 his 3-11,15 leu2-3, 112 trp1-1 ura3 sml1-1 rad53-XB::HIS3 Δmec1::TRP DDC2 FLAG::LEU::ddc2 | Our laboratory |
| yJKD 425 | W303 | MATaade2-1 can1-100 his 3-11,15 leu2-3, 112 trp1-1 ura3 sml1-1 rad53-XB::HIS3 Δmec1::TRP Δtel1::KANR | Our laboratory |
| DLY408 | W303 | MATabar1::HISG cdc13-1 cdc15-2 ade2-1 can1-100 his 3-11,15 leu2-3, 112 trp1-1 ura3 GAL+ psi+ ssd1-d2 | T. Weinert |
| DLY409 | W303 | MATabar1::HISG cdc13-1 cdc15-2 rad9::HIS3 ade2-1 can1-100 his 3-11,15 leu2-3, 112 trp1-1 ura3 GAL+ psi+ ssd1-d2 | T. Weinert |
| yJKD 007 | W303 | MATabar1::HISG cdc13-1 cdc15-2 ade2-1 can1-100 his 3-11,15 leu2-3, 112 trp1-1 ura3 GAL+ psi+ ssd1-d2 sml1D::TRP1 rad53Δ::HIS3 Δrad9 pipo | Our laboratory |
| yJKD 103 | W303 | MATabar1::HISG cdc13-1 cdc15-2 ade2-1 can1-100 his 3-11,15 leu2-3, 112 trp1-1 ura3 GAL+ psi+ ssd1-d2 sml1D::TRP1 rad53Δ::HIS3 | Our laboratory |
| yJKD 014 | W303 | MATabar1::HISG cdc15-2 ade2-1 can1-100 his 3-11,15 leu2-3, 112 trp1-1 ura3 GAL+ psi+ ssd1-d2 sml1D::TRP1 rad53Δ::HIS3 | Our laboratory |
| yJKD 016 | W303 | MATabar1::HISG cdc15-2 ade2-1 can1-100 his 3-11,15 leu2-3, 112 trp1-1 ura3 GAL+ psi+ ssd1-d2 sml1D::TRP1 rad53Δ::HIS3 Δrad9 pipo | Our laboratory |
| Y1130 | W303 | MATaade2-1 can1-100 trp1-1 leu2-3112 his3-11,15 ura3 + [pBAD070]** | S. Elledge |
| Y1131 | W303 | MATaade2-1 can1-100 trp1-1 leu2-3112 his3-11,15 ura3 rad9Δ::his5+ mrc1Δ-2::HIS3 + [pBAD070] | S. Elledge |
| ySJL340-1 | W303 | MATaade2-1 can1-100 trp1-1 leu2-3112 his3-11,15 ura3 DUN1-13 × MYC::LEU::dun1 + [pBAD070]** | Our laboratory |
| ySJL342-1 | W303 | MATaade2-1 can1-100 trp1-1 leu2-3112 his3-11,15 ura3 rad9Δ::his5+ mrc1Δ-2::HIS3 DUN1-13 × MYC::LEU::dun1 + [pBAD070] | Our laboratory |
| ySJL361-1 | W303 | MATaade2-1 can1-100 trp1-1 leu2-3112 his3-11,15 ura3 DUN1-13 × MYC::LEU::dun1 DDC2-RAD53::URA::rad53 + [pBAD070] | Our laboratory |
| ySJL364-7 | W303 | MATaade2-1 can1-100 trp1-1 leu2-3112 his3-11,15 ura3 rad9Δ::his5+ mrc1Δ-2::HIS3 DUN1-13 × MYC::LEU::dun1 DDC2-RAD53::URA::rad53 + [pBAD070] | Our laboratory |
| yJS422 | W303 | MATaade2-1 can1-100 trp1-1 leu2-3112 his3-11,15 ura3 Δddc2::KANR + [pBAD070] | Our laboratory |
| yJS424 | W303 | MATaade2-1 can1-100 trp1-1 leu2-3112 his3-11,15 ura3 rad9Δ::his5+ mrc1Δ-2::HIS3 Δddc2::KANR + [pBAD070] | Our laboratory |
| YLL334 | W303 | MATaade2-1 trp1-1 leu2-3112 his3-11,15 ura3 can1-100 HA2-DDC1::LEU2::ddc1 | M. P. Longhese |
| DMP 2161/25B | W303 | MATaa ade2-1 trp1-1 leu2-3112 his3-11,15 ura3 can1-100 | M. P. Longhese |
| HA2-DDC1::LEU2::ddc1 rad17Δ::LEU2 rad24Δ::TRP1 mec3Δ::TRP1 | |||
| ySJL351-5 | W303 | MATaa ade2-1 trp1-1 leu2-3112 his3-11,15 ura3 can1-100 | Our laboratory |
| HA2-DDC1::LEU2::ddc1 rad17Δ::LEU2 rad24Δ::TRP1 mec3Δ::TRP1 DDC2-RAD53::URA::rad53 | |||
| W1588-4C | W303 | MATaa ade2-1 can1-100 trp1-1 leu2-3112 his3-11,15 ura3 | R. Rothstein |
**
pBAD070-relevant markers: Apr TRP1 GAP-RNR1