Table 2.
Plasmids used in this study
| Name | Abbreviation | Description | Source |
|---|---|---|---|
| pRS316 RAD53 3×FLAG | pRAD53 F | RAD53 3 × FLAG under endogenous Rad53 promoter | Our laboratory |
| pRS316 rad53 kDa 3×FLAG | prad53 kDa F | rad53 kDa 3 × FLAG under endogenous Rad53 promoter | Our laboratory |
| pRS315 RAD53 2 ×HA | pRAD53 H | RAD53 2 × HA under endogenous Rad53 promoter | Our laboratory |
| pRS305 DUN1 13×MYC | DUN1 13 × MYC genomic integration plasmid Cut Nco I to target | Our laboratory | |
| pRS315 MYC-MEC1 | pMEC1 | 18 × MYC MEC1 under endogenous Mec1 promoter | Our laboratory |
| pRS316 DDC2-RAD53 3× FLAG | pD2-R53 F | DDC2 ORF fused with RAD53 3 × FLAG at the 5′end of RAD53 under Rad53 promoter | Our laboratory |
| pRS316 DDC2-rad53 kDa 3× FLAG | pD2-r53 kDa F | DDC2 ORF fused with rad53 kDa 3 × FLAG at the 5′end of RAD53 under Rad53 promoter | Our laboratory |
| pRS315 DDC2-RAD53 2 × HA | pD2-R53 H | DDC2 ORF fused with RAD53 2 × HA at the 5′end of RAD53 under Rad53 promoter | Our laboratory |
| pRS306 DDC2-RAD53 | DDC2-RAD53 genomic integration plasmid Cut SexA I to target | Our laboratory | |
| pRS316 DDC2 3× FLAG | pD2 F | DDC2 ORF 3 × FLAG under endogenous Rad53 promoter and Rad53 3′UTR | Our laboratory |
| pRS316 DDC2-3× HA | pD2 H | DDC2 ORF 3 × HA under endogenous DDC2 promoter and ADH1 terminator | Our laboratory |
| pRS305 DDC2-3 × FLAG | DDC2 genomic integration plasmid Cut Ndel to target | Our laboratory | |
| pRS316 ddc2KA 3× FLAG | pd2KA F | ddc2KA(ddc2 K177A, K178A) 3 × FLAG under endogenous Rad53 promoter and Rad53 3′UTR | Our laboratory |
| pGEX 4T2 DDC2 | pGST-DDC2 | Bacterial expression vector for GST-Ddc2 | Our laboratory |