Table 1.
Yeast two-hybrid analysis of interactions of the PAKc CRIB/AI domain with RacB
| A.
|
|
|---|---|
| RacB
|
PAKc
|
| CRIB/AI | |
| RacB G12V | +++++ |
| RacB Q61L | +++++ |
| RacB N17S | - |
| B.
|
|
|---|---|
| CRIB/AI
|
Interaction
|
| RacB Q61L | |
| Wild-type | +++++ |
| L144F | +++ |
| H121,124L | - |
A. Relative interactions between constitutively active (RacBG12V and RacBQ61L) and dominant negative (RacBN17S) forms of RacB and the CRIB and autoinhibitory domain (CRIB/AI).
B. Relative interactions between RacBQ61L and wild-type CRIB/AI and mutant forms of the CRIB/AI domain.