Table 2.

DIAS analysis of chemotaxis

Vector			PAKc	PAKc	PAKc K234A Kinase dead	PAKc R34C	PAKc S466A, P470A Gβγ “binding site”
Strain background	KAx-3	PAKc null	KAx-3	PAKc null	KAx-3	KAx-3	KAx-3
Speed (μm/min)	9.96 ± 0.70	8.92 ± 0.55	5.58 ± 0.77	6.94 ± 0.83	3.70 ± 0.37	11.3 ± 0.01	9.18 ± 2.04
Dir ch (deg)	24.1 ± 4.0	29.3 ± 1.3	55.8 ± 1.65	55.8 ± 11.2	38.6 ± 4.21	19.6 ± 1.71	25.1 ± 7.39
Roundness	50.4 ± 3.0	68.2 ± 5.0	44.5 ± 2.04	59.9 ± 3.66	57.7 ± 2.92	59.2 ± 2.00	63.7 ± 5.46
Directionality	0.78 ± 0.01	0.73 ± 0.03	0.43 ± 0.05	0.48 ± 0.11	0.59 ± 0.04	0.81 ± 0.01	0.74 ± 0.09

Vector	PAKc ΔPH domain	PAKc H121, 124L	PAKc L144F	PAKc ΔPH domain	PAKc H121, 124L	PAKc L144F
Strain background	KAx3	KAx-3	KAx-3	PAKc null	PAKc null	PAKc null	MIHCK null	MIHCK/PAKc null
Speed (μm/min)	7.98 ± 0.79	6.36 ± 0.51	11.5 ± 0.48	9.45 ± 0.75	8.13 ± 0.35	11.1 ± 0.68	9.55 ± 0.70	3.49 ± 0.07
Dir ch (deg)	37.0 ± 11.4	34.5 ± 1.17	28.2 ± 1.71	28.1 ± 8.28	51.2 ± 2.15	43.6 ± 11.4	23.6 ± 2.94	62.2 ± 5.02
Roundness	48.6 ± 4.81	58.4 ± 3.07	72.1 ± 2.28	58.9 ± 4.06	48.2 ± 0.65	47.7 ± 6.17	60.0 ± 0.36	64.0 ± 5.01
Directionality	0.67 ± 0.09	0.71 ± 0.01	0.75 ± 0.01	0.79 ± 0.04	0.49 ± 0.01	0.57 ± 0.06	0.81 ± 0.10	0.33 ± 0.07

DIAS analysis of chemotaxis. Numbers are mean values ± standard deviation. Dir ch, direction of change of direction. Speed indicates speed of cell's centroid movement along the total path. Direction change is a relative measure of the number and frequency of turns the cell makes. Larger numbers indicate more turns and less efficient chemotaxis. Directionality is a measure of the linearity of the pathway. Cells moving in a straight line to the needle have a directionality of 1.00. Roundness is an indication of the polarity of the cells. Larger numbers indicate the cells are more round (less polarized).