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. 2004 Dec;15(12):5551–5564. doi: 10.1091/mbc.E04-07-0640

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Copyright © 2004, The American Society for Cell Biology

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Figure 1. — Composition of S. pombe septin complexes. (A–H) Silver-stained gels of a portion of Spn-TAP complexes from the indicated strains. Spn proteins are indicated with arrows. Note that the calmodulin-binding portion of the TAP tag remains after the purification and changes the mass of the proteins. Other bands are nonspecific contaminants. (I) Protein lysates prepared in denaturing conditions from the indicated strains were resolved by SDS-PAGE and blotted with anti-GFP serum. (J) anti-myc (top panel) and anti-HA (bottom panel) immunoprecipitates from the indicated strains were blotted with anti-myc antibodies.