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. 2004 Dec;15(12):5551–5564. doi: 10.1091/mbc.E04-07-0640

Figure 7.

Figure 7.

Septin recruitment to the cortex, but not stable ring formation, can be induced during interphase. (A) spn3-GFP cdc16-116 (KGY738) and (B) mid2-GFP cdc16-116 (KGY741) cells were grown to midlog phase at 25°C, synchronized in early G2 phase by centrifugal elutriation, and then shifted to 36°C. Samples of cells were collected every 15 min. One portion of each sample was imaged immediately to determine the percentage of septated cells and the percentage of cells with Spn3p-GFP and Mid2p-GFP medial localization, respectively. Another portion was fixed with ethanol immediately and then stained with DAPI to determine the percentage of binucleate cells. (C and D) Spn3p-GFP localization in live cells from (C) 60 min or (D) 150 min time points (left panels of each). Images in the left panels were also rotated in the Z-axis to better reveal septin ring organization (right panels). Short arrows point to disorganized septin rings at 60 min. Longer arrows point to organized rings at 150 min. (E) Mid2p-GFP localization in live cells from 150 min time point. Cells are also rotated in the Z-axis to better show Mid2p ring organization. Arrows point to organized Mid2p rings. Scale bar, 5 μm.