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. 2004 Dec;15(12):5574–5582. doi: 10.1091/mbc.E04-02-0142

Figure 11.

Figure 11.

(A) Hydrogen peroxide activation of RLM1 transcription in mpk1Δ strain. Wild-type strain (Dl100) (▪) and the isogenic mpk1Δ (▴) strain were transformed with px2RLM1 and then plated in 24-well plates on media containing the β-galactosidase substrate CPRG as described in Materials and Methods. Hydrogen peroxide was added to the media in increasing concentrations (0.1–0.5 mM), and β-galactosidase activity was measured at 570 nm after 24 h. Results shown are from five independent assays. (B) RLM1 transcription induction in hog1Δ strain. Wild-type strain (EY957) (▪) and the isogenic hog1Δ strain (•) were transformed with px2RLM1 and then plated in 24-well plates on media containing the β-galactosidase substrate CPRG as described in Materials and Methods. Hydrogen peroxide was added to the media in increasing concentrations (0.1–0.5 mM), and β-galactosidase activity was measured at 570 nm after 24 h. Results shown are from five independent assays.