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. 2004 Dec;15(12):5574–5582. doi: 10.1091/mbc.E04-02-0142

Figure 2.

Figure 2.

(A) Catecholamines activate FUS1 reporter in a dose-dependent manner. The wild-type yeast strain (EY957) was plated in 24-well plates on medium containing the β-galactosidase substrate CPRG as described in Materials and Methods. Catecholamines (adrenaline [×], noradrenaline [▴], l-dopa [▪], and dopamine [*]) were added to the media in increasing concentrations (0.01–1 mM) and β-galactosidase activity was measured at 570 nm after 48 h. Results shown are from five independent assays. (B) l-Dopa activates RLM1 reporter in a dose-dependent manner. Wild-type strain (EY957) transformed with px2RLM1 was plated in 24-well plates on media containing the β-galactosidase substrate CPRG as described in Materials and Methods. Catecholamines (adrenaline [×], noradrenaline [▴], l-dopa [▪], and dopamine ([988]) were added to the media in increasing concentrations (0.01–1 mM) and β-galactosidase activity was measured at 570 nm after 48 h. Results shown are from five independent assays.