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N-Acetyl-cysteine reverses the l-dopa–induced activation of the reporter FUS1. The wild-type yeast strain (EY957) was plated in 24-well plates, treated with l-dopa in different concentrations and N-acetyl-cysteine. Shown are l-dopa (0.02 mM) and N-acetyl-cysteine (2 mM) alone or in combination, and β-galactosidase activity was measured as described in Materials and Methods. Results shown are from five independent assays.
