Figure 9.

(A) RLM1 transcription in treated with l-dopa kss1Δfus3Δ strain. Wild-type strain (EY957) (▪) and the isogenic kss1Δfus3Δ (♦) strain were transformed with px2RLM1 and then plated in 24-well plates on media containing the β-galactosidase substrate CPRG as described in Materials and Methods. l-Dopa was added to the media in increasing concentrations (0.01–0.5 mM), and β-galactosidase activity was measured at 570 nm after 24 h. Results shown are from five independent assays. (B) FUS1 transcription in mpk1Δ strain. Wild-type strain (DL100) (▪) and the isogenic mpk1Δ (▴) strain were transformed with px2RLM1 and then plated in 24-well plates on media containing the β-galactosidase substrate CPRG as described in Materials and Methods. l-Dopa was added to the media in increasing concentrations (0.01–0.5 mM), and β-galactosidase activity was measured at 570 nm after 24 h. Results shown are from five independent assays