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Growth phenotypes. Haploid wild-type and mutant strains expressing Tub2, Tub2-1×TetCys, or Tub2-2×TetCys, respectively, were grown overnight in glucose-containing medium. Then, 10-fold serial dilutions were spotted onto YPD-plates. The plates were incubated for 5 d at different temperatures. (A) 30°C. (B) 25°C. (C) 37°C.
