Table 1.

Oligonucleotides used for construction and verification of tagged strains

Name	Sequence
TUB2-for	5′-GCTCCACAAAACCAAGATGAACCAATCACTGAGAATTTTGAAGGATCCTCTGGATGTTGTCCT-3′
TUB2-rev	5′-CCGCTGAAGTGAGGATCAAAAATCAATAGCTCGGAAGGTTAAAGGACTATAGGGAGACCGGCAGAT-3′
TUB2-3′ out	5′-ATTTGAACAACTTGGTCTCG-3′
ATP1-for	5′-GGCATCTCTAAAGAGTGCTACTGAATCATTTGTTGCCACTTTTGGATCCTCTGGATGTTGTCCT-3′
ATP1-rev	5′-GATTTTTCAGGGTTATTGTTGGGCTGCACTTTAAATTTAGTACTATAGGGAGACCGGCAGAT-3′
ATP1-3′ out	5′-CTGAAGCCGCTCCTCTACAAT-3′
kanB	5′-CTGCAGCGAGGAGCCGTAAT-3′
pU-R0	5′-TATTCTGGGCCTCCATGTC-3′