Figure 11.
Soluble heparin and U0126 impede the ability of Ccn2+/+ mouse embryonic fibroblasts to spread on fibronectin. Experiments were performed essentially as described in the legend to Figure 9. Ccn2+/+ mouse embryonic fibroblasts were allowed to adhere to fibronectin for up to 120 min (min on FN) as described in Materials and Methods, in the presence of soluble heparin (50 μg/ml) or U0126 (10 μM). Cells were fixed after 45 min and subjected to indirect immunofluorescence analysis with an anti-α-SMA antibody and an appropriate FITC-conjugated secondary antibody. Nuclei were detected by DAPI staining (blue).