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CRISPR/Cas9 system targeting pig GGTA1 and CMAH loci. (A) Schematic of sgRNAs targeting GGTA1 and CMAH loci. The target sgRNAs were designed in the last exon of the GGTA1 locus and the first exon of the CMAH locus. sgRNA-target sequences are labeled in lowercase and the protospacer adjacent motif (PAM) is underlined. (B) Surveyor assay (T7E1) for Cas9-mediated cleavage at GGTA1 and CMAH loci in PK15 cells.
