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. 2016 Oct 8;63(1):17–26. doi: 10.1262/jrd.2016-079

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©2017 Society for Reproduction and Development

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: http://creativecommons.org/licenses/by-nc-nd/4.0/)

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Fig. 4. — Immunofluorescence and flow cytometry analysis of Gal and Neu5Gc expression in GGTA1/CMAH DKO pigs. (A–B) Immunofluorescence of various tissues from wild-type or GGTA1/CMAH DKO pigs stained with anti-Neu5Gc antibody (A) and BS-IB4 lectin (B) (Magnification × 200; Neu5Gc, red; Gal, green; Nuclei, blue). (C–D) Flow cytometry analysis of AECs (C) and PBMCs (D) from the pigs indicated in A-B stained with anti-Neu5Gc antibody and BS-IB4 lectin. Human umbilical vein endothelial cells (HUVECs) and human PBMCs (HPBMCs) were negative controls, respectively. The green lines represent negative controls; the red lines represent the experimental groups.