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Human antibody binding and antibody-mediated complement-dependent cytotoxicity of cells from GGTA1/CMAH DKO pigs. (A–B) Flow cytometry analysis of human IgM and IgG binding to RBCs (A) and PBMCs (B) from wild-type (n = 3), GGTA1-KO (n = 3), and GGTA1/CMAH DKO (n = 3) pigs. (C) Flow cytometry analysis of cytotoxicity of PBMCs from GGTA1-KO (n = 3) and GGTA1/CMAH DKO (n = 3) pigs incubated with different human serum concentrations. (*P < 0.05, ** P < 0.01, *** P < 0.001).
