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. 2016 Oct 15;63(1):27–36. doi: 10.1262/jrd.2016-111

Fig. 1.

Fig. 1.

Cell morphology, hTERT mRNA detection, and telomere length in GGCs and hTERT-GGCs. (A) Morphology of GGCs at passage 3, and hTERT-GGCs at passage 30 and 50. Bar = 100 μm. (B) Immunofluorescence detection of the hTERT protein in GGCs and hTERT-GGCs. Immunofluorescence staining analysis of GGCs at passage 3, hTERT-GGCs at passage 30, and hTERT-GGCs at passage 50 were performed using hTERT antibodies. Bar = 50 μm. (C) RT-PCR analyses confirming the expression of hTERT mRNA. GGCs at passage 3 in lane 1, hTERT-GGC at passage 30 in lane 2, hTERT-GGCs at passage 50 in lane 3, and HeLa cells as positive controls in lane 4. (D) The relative telomere length detection by quantitative PCR. The telomere length is measured as the ratio of telomere copy number/ single copy gene copy number (T/S). For each sample, the values of T and S are measured by the standard curve method. The T/S ratio reflects the length of the telomeres. Data are presented as means ± SEM of three independent experiments. Bars with different letters are significantly different (P < 0.05).