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. 2016 Oct 15;63(1):27–36. doi: 10.1262/jrd.2016-111

Fig. 2.

Fig. 2.

The detection of proliferation, cell cycle, and steroidogenic activity in hTERT-GGCs. (A) Growth curves of GGCs and hTERT-GGCs as determined by the CCK-8 method. Data presented are means ± SEM of three independent experiments. (B) Cell cycle distribution of GGCs at passage 7 and hTERT-GGCs at passage 50 as detected by flow cytometry. The percentage of hTERT-GGCs in S-phase is significantly higher than that of GGCs under the same culture conditions. (C and D) The 24 h secretion of estradiol and progesterone in GGCs and hTERT-GGCs was measured using an ELISA kit. Androstenedione was added to the culture medium and served as the substrate, followed by stimulation with 0, 0.5, or 5 IU/ml FSH. Data are presented as means ± SEM of three independent experiments. Bars with different letters are significantly different (P < 0.05).