Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Oct 15;63(1):27–36. doi: 10.1262/jrd.2016-111

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

©2017 Society for Reproduction and Development

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: http://creativecommons.org/licenses/by-nc-nd/4.0/)

PMC Copyright notice

Fig. 3. — Steroidogenesis-related gene expression, karyotype, and tumorigenicity in hTERT-GGCs. (A) Phenotypic features of hTERT-GGCs. The expression of some key genes related to steroidogenesis was measured by RT-PCR. GGCs at passage 5 in lane 1, hTERT-GGCs at passage 30 in lane 2, and hTERT-GGCs at passage 50 in lane 3. GAPDH was used as an internal control. (B) Karyotype analysis of hTERT-GGCs at passage 50. The chromosomal rearrangements of hTERT-GGCs showed the normal karyotype for goat. (C) Soft agar colony comparison between hTERT-GGCs at passage 50 (i) and Sp2/0 (ii). (i) No colonies were formed in hTERT-GGCs over 10 days of culture. (ii) Colony formation can be observed after 10 days of culture from Sp2/0 cells.