Table 1. Primer pairs used for PCR and Real time PCR.
| Gene | Sequences (5ˊ→3ˊ) | Product length (bp) |
Annealing temperature (°C) |
References or GenBank accession number |
| TERT | Forward: tatgccgtggtccagaagg | 384 | 56 | [33] |
| Reverse: caagaaatcatccaccaaacg | ||||
| StAR 1 | Forward: tactaaaggagccgtggataaag | 576 | 56 | XM_005698829 |
| Reverse: ctacaagtggtaatggttgggtt | ||||
| LHCGR | Forward: ctctttctttgccatctcag | 270 | 58 | [39] |
| Reverse: cgggagggcttatttga | ||||
| CYP19A1 | Forward: ggcatcatatttaacaatccagca | 547 | 60 | [53] |
| Reverse: cagacatggtgtctggcgctgcgatca | ||||
| CYP11A1 1 | Forward: gcagagggcgacataagca | 233 | 57 | [30] |
| Reverse: ggtcacggagatagggtgga | ||||
| CYP17A1 | Forward: tttcaaggtgaagatcgaggtg | 146 | 58 | [30] |
| Reverse: cggggaggaagaaggaatg | ||||
| ESR1 | Forward: caagaaggtccaatccgagt | 846 | 55 | XM_013966607.1 |
| Reverse: aaagagcagtttccgtgaac | ||||
| ESR2 | Forward: tccattgccagtcgtcacttc | 634 | 60 | NM_001285688.1 |
| Reverse: gccagcttggtcaaggacatc | ||||
| FSHR | Forward: gggctgggtctttgcttttg | 494 | 60 | KJ817181.1 |
| Reverse: ctgacctgtaggtctgggct | ||||
| Telomere | Forward: ggtttttgagggtgagggtgagggtgagggtgagggt | 39 | 58 | [50] |
| Reverse: tcccgactatccctatccctatccctatccctatcccta | ||||
| GAPDH 1 | Forward: tctgctgatgcccccatgtt | 289 | 56 | [48] |
| Reverse: tgaccttgcccacagccttg | ||||
| GAPDH 2 | Forward: gatggtgaaggtcggagtgaac | 100 | 56 | [5] |
| Reverse: gtcattgatggcgacgatgt | ||||
| StAR2 | Forward: aggccatgggcgagtggaac | 145 | 60 | XM_005698829.1 |
| Reverse: gtacagcgcacgctcacaaa | ||||
| CYP11A1 2 | Forward: tgatggctccagaggcaataaa | 150 | 60 | NM_001287574.1 |
| Reverse: caaaggcaaagtgaaacaggtc | ||||
| 3β-HSD | Forward: tccacaccagcaccatagag | 143 | 60 | NM_001285716.1 |
| Reverse: ttccagcacagccttctcg |
The numbers (in bp) in the right margin indicate the sizes of the expected amplified products for each set of primers. GAPDH1 was used as an internal control in PCR, GAPDH2 was used as an internal control in Real time PCR.