Fig. 3.

Analysis of CpG methylation in the Prop1 region during pituitary development. DNA methylation status of each PCR clone was determined by sodium bisulfite sequencing of genomic DNA samples prepared from rat pituitary glands on E13.5, E14.5, P4, and P30 and rat livers on E13.5, P4, and P30. Anterior and intermediate/posterior lobes were separately collected from postnatal pituitary glands on P4 and P30. The open and closed circles on the grids indicate unmethylated and methylated CpG sites, respectively, and numbers indicate the percentage of the CpG sites methylated in each region. Boxed CpG sites are hypomethylated in E13.5 pituitary glands, DNA samples as compared with E13.5 liver DNA samples, where the difference is > 30%.