. 2016 Oct 21;63(1):37–44. doi: 10.1262/jrd.2016-102

Table 1. List of primer sets specific for bisulfite-converted genomic DNAs.

Target		Sequence (5'–3')	Annealing temperature
Region 1	Forward	GGAGAAGATTTTAGTAAGATATGGGGGT	58°C
	Reverse	CTCCAATACCAATAACACAACATCCAATA	58°C

Region 2	Forward	GGATGTTGTGTTATTGGTATTGGAGTT	60°C
	Reverse	TACCAAAATCCACAACAAAACTCCTA	60°C

Region 3	Forward	ATTGGTTGAATGAAGAAATTAGAGAGAT	60°C
	Reverse	CACCTCTCCTCTAAACCTCACTA	60°C

Region 4	Forward	TTAAGTTTGGGGGTTTTGGTGATT	62°C
	Reverse	AAACCCACATCTACTACTCCCTCAA	62°C

Region 5	Forward	GGAGGGTTGTTTAGTATTTGTTTGAAGAT	60°C
	Reverse	CACAACACCTCCCCACCCTAA	60°C

Region 6	Forward	AGAAGGAGAGATATAAGAGGAGGTGT	60°C
	Reverse	TAAATCTCCCCAACCCTATACCAA	60°C

Region 7	Forward	GGTATAGGGTTGGGGAGATTTAAGT	60°C
	Reverse	CACCTCTCCAAACCTACATCCAAA	60°C