Fig. 2.

Incorporation of exosome-like vesicles into MGCs and cumulus cells in vitro. MGCs (A–D) and COCs (E–G) were cultured with PKH67-labeled exosome-like vesicles for 24 h, and observed using a confocal fluorescence microscope. MGCs and COCs were counterstained with PI (B) and DAPI (F), respectively. Bars indicate 10 μm (A–D) and 20 μm (E–G). A z-stack image was generated from the cells shown in A–C by collecting a series of images captured at 0.52-µm thickness intervals (D). Arrowheads indicate PKH67-labeled exosome-like vesicles that were taken up by MGCs (A–D) and cumulus cells (E–G).