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. 2017 Feb 5;63(1):51–58. doi: 10.1262/jrd.2016-124

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Fig. 4. — Effect of exosome-like vesicles on cumulus expansion in mouse COCs. (A) Representative photographs of mouse COCs cultured with or without the exosomal fraction (Exo) in the absence or the presence of FSH or EGF for 14 h. Bar, 100 µm. (B) Expression levels of the cumulus expansion-related transcripts (Has2, Ptgs2, and Tnfaip6) in mouse COCs cultured for 6 h with or without the exosomal fraction (Exo) in the absence (black bars) or presence of FSH (white bars) or EGF (gray bars). Values are shown as the means ± SEM of independent experiments repeated 3 times. The mean values of the expression levels in COCs not treated with the exosomal fraction, FSH, or EGF were set to 1. n.s., not significant.