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. 2016 Nov 28;63(1):111–115. doi: 10.1262/jrd.2016-144

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©2017 Society for Reproduction and Development

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: http://creativecommons.org/licenses/by-nc-nd/4.0/)

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Fig. 2. — Anti-sperm antibody titers in serum of male rats after immunization with sperm and adjuvant in the immature period. Values are shown as counts per second (cps). In the control groups (A), means ± SEM are shown. Non-treated (○) and FCA (□) and ISA 71VG + LPS (◊) groups. In the FCA-treated group, a significant difference from the other two groups was detected at 15 weeks of age. In the sperm-immunized groups (B, C and D), individual data are shown. Rats were immunized with sperm and ISA 71VG (B), sperm and FCA (C) or sperm and ISA 71VG supplemented with LPS (D). Data for males that were sterile at 20 weeks of age are shown with filled markers. Dashed lines in B, C and D show mean + 3 × SEM of the non-treated group.