. 2016 Nov 28;63(1):111–115. doi: 10.1262/jrd.2016-144

Table 1. Effects of sperm inoculation on fertility in male rats.

Treatment*		n	Fertility^** (10 weeks of age)		Fertility ** (20 weeks of age)			Testis weight^*** (g)
adjuvant	sperm	n	/all^†	/ fertile^†† (n)	/all^†	/ fertile^†† (n)	sterile males (%)	/all^†	/fertile (n) ^††	/ sterile (n) ^††
none	–	4	16.0 ± 0.50	16.0 ± 0.50 (4)	14.1 ± 0.34 ^ab	14.1 ± 0.34 (4) ^a	0/4 (0)	1.51 ± 0.024 ^a	1.51 ± 0.024 (4) ^a	-
FCA	–	4	16.4 ± 1.38	16.4 ± 1.38 (4)	14.8 ± 0.63 ^a	14.8 ± 0.63 (4) ^a	0/4 (0)	1.43 ± 0.034 ^a	1.43 ± 0.034 (4) ^ab	-
ISA 71VG + LPS	–	4	14.4 ± 0.80	14.4 ± 0.80 (4)	15.0 ± 0.84 ^a	15.0 ± 0.84 (4) ^a	0/4 (0)	1.59 ± 0.007 ^a	1.59 ± 0.007 (4) ^a	-
FCA	+	8	13.3 ± 1.94	15.2 ± 0.42 (7)	4.8 ± 2.50 ^bc	14.2 ± 1.42 (3) ^a	5/8 (62.5)	0.83 ± 0.100 ^b	1.20 ± 0.123 (3) ^ab	0.60 ± 0.018 (5)
ISA 71VG	+	4	10.1 ± 3.38	13.5 ± 0.00 (3)	11.0 ± 3.67 ^ac	14.7 ± 0.33 (3) ^a	1/4 (25.0)	1.12 ± 0.229 ^a	1.34 ± 0.186 (3) ^ab	0.48 (1)
ISA 71VG + LPS	+	8	11.0 ± 2.43	14.7 ± 0.56 (6)	3.1 ± 1.61 ^c	8.3 ± 1.59 (3) ^b	5/8 (62.5)	0.76 ± 0.062 ^b	0.94 ± 0.094 (3) ^b	0.65 ± 0.046 (5)

* Each male was administered adjuvant with/without sperm (2 × 10⁷) at 2 and 4 weeks of age. ** Fertility was examined by mating tests. Each male was placed with a pro-estrous female and the female was examined for the number of fetuses at 12–14 days after mating. *** Testicular weights were measured at 21–22 weeks of age, and a few at 30 weeks of age. ^† Means ± SEM were calculated from numbers of males in column “n”. ^†† Means ± SEM were calculated from the numbers of fertile males indicated in parentheses. ^a–c Values with different superscripts are significantly different (P < 0.05).