Figure 1. Length distribution and different classes of sequence reads identified in the small RNA libraries of C. roseus.

(A) Size distribution of the total reads. (B) Size distribution of unique reads. (C) Distribution of different classes of sequence reads derived from the small RNA libraries. Putative miRNAs and un-annotated sRNAs constitute 60–75% of the total cleaned reads in all twelve libraries. Each small RNA library (control, 1 h-, 8 h-, or 24 h-MeJA treated) is represented by three biological replicates. C. roseus seedlings treated with 100 μM MeJA for 1 h, 8 h, or 24 h were used for RNA isolation and library preparation. Mock-treated seedlings served as control.