Table 2. Kinetic parameters derived from sensograms of Con A binding to either SKBR3 cell surfaces or rHER2 protein using TraceDrawer software, either using 1:1 simple or 1:2 binding models.
| SKBR-3 CELLS | ||||
|---|---|---|---|---|
| ka (M−1s−1) | kd (s−1) | KD (nM) | Bmax | |
| Untreated | 2.46 E+4 (±11.92) | 8.9 E−6 (±6.23 E−5) | 0.362 (±0.07) | 125.8 (±0.007) |
| 20 mM sodium periodate | 5.8 E+4 (±1.49 E+3) | 1.93 E−3 (±1.08 E−5) | 32.8 (±1.02) | 14.24 (±0.022) |
| rHER2 PROTEIN | ||||
| Untreated | 1.75 E+5 (±3.62 E+1) | 8.76 E−4 (±1.48 E−6) | 5.02 (±0.03) | 11.17 (±0.08) |
| 20 mM sodium periodate | 1.51 E+5 (±2.85 E+2) | 1.89 E−4 (±5.12 E−7) | 1.25 (±0.004) | 5.03 (±0.05) |
Mean average values ± SD, derived from two sensor chips for each condition and four analyte concentrations.