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. 2017 Feb 22;7:42996. doi: 10.1038/srep42996

Figure 1. Successful isolation of exosomes from DCs culture medium.

Figure 1

(A) The ultrastructure of exosome by transmission electron microscopy. Bar size, 100 nm. (B) The size distribution profile of immature and mature DC-exos by Nanosight, revealing a size peak of 113 nm in immature DC-exos and 109 nm in mature DC-exos. (C) The expression of exosomes negative marker, Calnexin and positive markers, Alix, CD63 and TSG101. And also, the expression of CCR7 was detected in both cell lysis and exosomes lysis. A total of 20ug protein from DCs lysis and 5 ug protein from exosomes lysis was loaded into each lane. Full-length blots can be found in Supplemental Figures 3 and 4.