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. 2017 Feb 22;7:42996. doi: 10.1038/srep42996

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(A) The PKH67-labelled (green) immature DC-exos and mature DC-exos were co-cultured with immature DCs for indicated time. Fee PKH67 dye was also incubated with immature DCs as a positive control. Then the DCs were fixed and stained with DAPI (blue). The uptake of DC-exos by DCs was observed under a confocal microscope. (B) Immature and mature DC-exos were co-cultured with immature DCs for 24 hours. Then the DCs were harvested for the detection of IL-1, IL-6 and TNF-α by quantitive PCR (n = 3). ***p < 0.001.