Table 2.

Quantitative analysis of probe nuclear localization

Code ID	DS1 axial pos (μm)	NE Dist Avg (μm)	fclose	Result (p < 0.001)	DS5 axial pos (μm)	NE Dist Avg (μm)	fclose	Result (p < 0.001)
1	-3.42	0.86	0.46	RAND	-2.78	0.87	0.47	RAND
2	-3.41	0.75	0.65	OUTER (p < 0.01)	-2.63	0.77	0.62	OUTER (p = 0.01)
3	-3.12	0.97	0.38	INNER (p = 0.01)	-2.46	0.89	0.44	RAND
4	-1.38	1.03	0.44	RAND	-0.95	0.89	0.49	RAND
5	-1.08	1.12	0.31	INNER	-0.71	1.11	0.27	INNER
6	-0.97	1.13	0.31	RAND	-0.67	0.97	0.38	INNER (p < 0.01)
7	0.09	1.02	0.38	INNER	0.12	0.93	0.44	RAND
8	0.51	1.03	0.34	INNER	0.39	0.91	0.40	RAND
9	0.75	1.00	0.39	RAND	0.46	0.98	0.40	RAND
10	2.40	0.90	0.47	RAND	1.64	0.98	0.36	INNER (p < 0.01)
11	2.54	0.88	0.43	RAND	1.80	0.98	0.35	INNER (p < 0.01)
12	2.78	0.89	0.42	RAND	2.00	0.91	0.39	RAND
13	3.97	1.26	0.17	INNER	3.32	1.30	0.16	INNER

Table 2 gives the average Rabl position and the average distance to the nuclear envelope for each of the 13 labels in DS1 and DS5. Additionally, for each labeled point, the statistically defined nuclear envelope localization is shown. For both datasets, the z averages increase along the chromosome, reflecting their polarized orientation (the average x and y Rabl coordinates are close to zero and not shown). In DS1 and DS5, the analysis shows one point (label 2) consistently closer to the nuclear envelope than the randomly generated points.