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. 2017 Feb 22;7:43146. doi: 10.1038/srep43146

Figure 3. Inhibition of FAK activation decreases ECM synthesis.

Figure 3

(a) Western blot evaluated expression of ECM including collagen-1, laminin, fibronectin, as well as LOX. (b) Densitometric analysis of blots for determining ECM normalized to GAPDH. (c) Cell culture medium supernatant was collected and analyzed using a Collagen I alpha 1 ELISA Kit, FAK inhibitor significantly decreased total collagen level in the hypoxia-cultured CFs compared with hypoxia-cultured CFs treated with vehicle only(514.35 ± 63.61 VS. 139.11 ± 48.57 pg/ml). (d) Densitometric analysis of blots for determining LOX to GAPDH. Multiple exposures of LOX are presented in Supplementary Figure S6; full-length blots are presented in Supplementary Figure S3. Data are presented as means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.