Table 1. Calibrated T_m values of 12 mutations detected using the MMCA assay.

	Color	Genotype*	Tm ± 3 SD	ΔTm ± 3 SDb
Reaction A	FAM	WT	65.4 ± 0.51	—
		c.35delG	60.9 ± 0.60	4.5 ± 0.60
	HEX	WT	64.0 ± 0.30	—
		c.176_191del16	61.5 ± 0.48	2.5 ± 0.52
	ROX	WT	59.9 ± 0.60	—
		c.299_300delAT	66.4 ± 0.58	6.5 ± 0.30
	ROX	WT	73.8 ± 0.72	—
		c.235delC	69.3 ± 0.82	4.5 ± 0.39
Reaction B	FAM	WT	66.8 ± 0.87	—
		m.1555A > G	63.3 ± 0.60	3.5 ± 0.64
	HEX	WT	63.5 ± 0.33	—
		m.1494C > T	56.5 ± 0.42	7.0 ± 0.42
	ROX	WT	63.3 ± 0.81	—
		m.7444G > A	59.5 ± 0.30	3.8 ± 0.66
		m.7445A > G	59.5 ± 0.31	3.8 ± 0.60
Reaction C	FAM	WT	65.6 ± 0.74	—
		c.919–2A > G	61.4 ± 0.66	4.2 ± 0.84
	HEX	WT	65.0 ± 0.30	—
		c.2168A > G	61.0 ± 0.54	4.0 ± 0.33
	ROX	WT	69.6 ± 0.51	—
		c.538C > T	63.1 ± 0.30	6.5 ± 0.45
		c.547G > A	65.6 ± 0.33	4.0 ± 0.60

^aWT, wild-type.

^bΔT_m = T_{m (wild-type)} − T_{m (mutant)}; —, not applicable.