Figure 5. Ficin applied to detect the H₂O₂ release from MCF-7 cells.

(a) Linearity of absorbance against a H₂O₂ concentration range of 0–20 μM. (b) Comparison of fluxes of H₂O₂ release from cells induced by ADP (200 ng mL⁻¹, 0.47 μM), AA (200 ng mL⁻¹, 1.14 μM), fMLP (200 ng mL⁻¹, 0.46 μM) and n-butyric acid (10 mM). All the conditions: 0.10 μg mL⁻¹ ficin, 0.80 mM TMB, 20 mM PBS buffer (pH 5.0), and 35 °C. Error bars represent the standard deviations of three independent experiments.