Table 1. Effects of P-CTX-1 on Na_V channel voltage dependence of activation and steady-state fast inactivation.

	P-CTX-1	Maximal Peak Current (I/Imax)	Activation		Inactivation
			V1/2 (mV)	k (mV)	V1/2 (mV)	k (mV)
NaV1.1	—	0.99 ± 0.00	−20.2 ± 0.4	4.5 ± 0.3	−51.8 ± 1.2	−6.7 ± 1.1
	+	0.95 ± 0.04	−25.3 ± 0.7*	4.7 ± 0.6	−53.8 ± 1.3	−6.9 ± 1.2
NaV1.2	—	1.00 ± 0.00	−20.6 ± 0.3	4.4 ± 0.3	−53.4 ± 0.6	−4.1 ± 0.5
	+	0.86 ± 0.09	−26.9 ± 0.8*	4.9 ± 0.7	−60.3 ± 1.2*	−5.6 ± 1.1*
NaV1.3	—	1.00 ± 0.00	−19.7 ± 0.3	4.9 ± 0.2	−58.0 ± 0.5	−4.1 ± 0.5
	+	1.08 ± 0.04	−24.7 ± 0.3*	3.9 ± 0.2*	−60.1 ± 0.6*	−4.2 ± 0.7
NaV1.4	—	0.93 ± 0.04	−36.8 ± 0.4	1.8 ± 0.3	−63.8 ± 0.5	−4.6 ± 0.4
	+	0.74 ± 0.16	−40.2 ± 0.8*	2.8 ± 0.7	−57.3 ± 1.5*	−5.8 ± 1.4
NaV1.5	—	0.93 ± 0.03	−37.6 ± 1.0	4.6 ± 0.9	−72.3 ± 0.6	−5.8 ± 0.6
	+	0.85 ± 0.07	−43.7 ± 1.3*	4.8 ± 1.1	−72.8 ± 0.8	−6.2 ± 0.7
NaV1.6	—	1.00 ± 0.00	−19.2 ± 0.2	4.8 ± 0.2	−56.0 ± 0.4	−5.1 ± 0.3
	+	1.11 ± 0.03*	−22.6 ± 0.2*	3.5 ± 0.2*	−57.1 ± 0.9	−5.4 ± 0.8
NaV1.7	—	0.91 ± 0.02	−33.2 ± 0.6	3.2 ± 0.5	−62.4 ± 0.4	−4.4 ± 0.4
	+	0.74 ± 0.10	−38.9 ± 1.5*	6.4 ± 1.4*	−64.4 ± 0.9	−5.7 ± 0.8
NaV1.8	—	0.94 ± 0.02	−5.6 ± 0.6	7.7 ± 0.6	−35.3 ± 1.7	−8.7 ± 1.5
	+	1.00 ± 0.08	−16.9 ± 0.3*	4.0 ± 0.2*	−36.1 ± 1.7	−7.9 ± 1.5
NaV1.9	—	0.99 ± 0.01	−48.3 ± 0.7	7.8 ± 0.6	−31.1 ± 2.1	−11.4 ± 1.6
	+	1.06 ± 0.15	−57.2 ± 1.1*	8.4 ± 1.0	−41.7 ± 2.6*	−12.7 ± 2.2

Electrophysiological parameters at hNa_V1.1–hNa_V1.9 heterologously expressed in HEK293 cells were assessed using whole-cell voltage-clamp recordings. Data are reported as mean ± SEM from 4–10 cells. *Indicates P < 0.05 compared to control.