Table 1.
Summary of speckle velocity and chromosome velocity data from cells injected at late prometa- or metaphase
| Stage analyzed | Method of analysis | Kinetochore microtubule, (kMT) speckles | Nonkinetochore microtubule, (nkMT) speckles | Half-bivalent chromosomes |
|---|---|---|---|---|
| Metaphase | Projections | 0.7 ± 0.3 μm/min | ||
| (n = 106) | ||||
| Manual tracking | 0.6 ± 0.2 μm/min | 0.8 ± 0.4 μm/min | ||
| (n = 43) | (n = 36) | |||
| Anaphase | Projections | 0.9 ± 0.2 μm/min | ||
| (n = 213) | ||||
| Manual tracking | 0.9 ± 0.2 μm/min | 0.9 ± 0.3 μm/min | 0.5 ± 0.1 μm/min | |
| (n = 31) | (n = 24) | (n = 25) | ||
| Anaphase half-bivalent velocity in uninjected control cells | 0.4 ± 0.1 μm/min | |||
| (n = 28) |
Student's t test: kMT anaphase projections vs. half-bivalent chromosomes: p < 0.0001; kMT anaphase projections vs. kMT metaphase projections: p < 0.0001; kMT anaphase manual vs. kMT metaphase manual: p < 0.0001; kMT metaphase manual vs. nkMT metaphase manual: p = 0.0015; kMT anaphase manual vs. nkMT metaphase manual: p = 0.19; nkMT metaphase manual vs. nkMT anaphase manual: p = 0.22; kMT anaphase manual vs. nkMT anaphase manual: p = 1.0