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. 2017 Feb 22;7:43081. doi: 10.1038/srep43081

Figure 6.

Figure 6

(A) HT29 and HCT116 cells were treated with plasma for 30, 60, and 120 sec, and whole-cell extracts were prepared, separated on SDS-PAGE, and subjected to Western blotting analysis against Sp1 antibody. Actin was employed as a loading control. The graphs indicate the ratio of Sp1–to-actin expression. (B) Effects of plasma (for 30, 60, and 120 sec) on Sp1 mRNA. The graphs indicate the ratio of Sp1-to-actin expression. Data represent mean percentage levels ± SD (n = 3; *p < 0.05). (C) Experiments to assess dose time–dependent effects of plasma on Sp1, caspase 3, cleaved caspase 3, PARP, and cleaved PARP were carried out using HT29 and HCT116 cells treated with plasma (120 sec) at 6, 12, 24, and 48 h. Actin was employed as a loading control. (D) Immunofluorescence microscopy of HT29 and HCT116 cells treated with plasma (for 30, 60, and 120 sec). Cells were immunostained with anti-Sp1, and signals were detected with 488-conjugated secondary antibody. DAPI was used for nuclear counterstaining. Data are expressed as means ± SD of three independent experiments, scale bar = 100 μm. (See color version online.).