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. 2017 Feb 22;7:42764. doi: 10.1038/srep42764

Figure 5. Palmitic acid induced apoptosis in podocytes.

Figure 5

Representative images of flow cytometry analysis of podocytes after treatment with different concentrations of palmitic acid for 24 hours. (a) Representative cytograms. (b) Percentage of apoptotic cells. ∗p < 0.05 vs. 0 μmol/L, #p < 0.05 vs. 50 μmol/L, Δp < 0.05 vs. 150 μmol/L. (c) Representative Western blot analyses of cleaved-caspase3 expression in podocytes treated with different concentrations of palmitic acid for 24 hours. (d) Densitometric analysis of cleaved-caspase3 expression in Figure c. ∗p < 0.05 vs. 0 μmol/L, #p < 0.05 vs. 50 μmol/L, Δp < 0.05 vs. 150 μmol/L. (e) Representative Western blot analyses of cleaved-caspase3 expression in podocytes treated with 150 μmol/L palmitic acid in the absence or presence of 150 μmol/L NAC for 24 hours. The cell lysates were analyzed by immunoblot using an antibody against cleaved-caspase3. (f) Densitometric analysis of cleaved-caspase3 expression in Figure e. The data are expressed as the mean ± SEM; n = 3. ∗p < 0.05 vs. control group, #p < 0.05 vs. NAC group, Δp < 0.05 vs. PA group. Ctr: Control group, podocytes were treated with 1% BSA. NAC: Podocytes were treated with 150 μmol/L N-acetyl-cysteine (NAC) for 24 hours. PA: Palmitic acid group, podocytes were treated with 150 μmol/L palmitic acid for 24 hours. PA + NAC: Podocytes were treated with 150 μmol/L palmitic acid for 24 hours after pretreatment with 150 μmol/L N-acetyl-cysteine (NAC) for 2 hours.