Figure 2. Time-lapse images of the sperm nuclear migration process after in vitro fusion of the tobacco sperm cell and egg cell induced in PEG medium.

(A–F) Images were taken at varying time intervals over a period of 30 min, as indicated in the bottom right corner of each image. Dotted circles indicate sperm nucleus location and arrowheads indicate the nucleus of egg cells in A–E. (F) The fertilized egg cell shows two nuclei stained by DAPI 30 min after membrane fusion. The smaller structure is the sperm nucleus and the bigger structure is the nucleus of the egg cell. Scale bar = 10 μm.