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. 2017 Feb 22;7:43161. doi: 10.1038/srep43161

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A–D) Time-lapse images of the sperm nuclear migration process after in vitro fusion of the maize sperm cell and egg cell after treatment with the microtubule-depolymerizing drug colchicine. Arrows indicate sperm cell’s contact with the egg cell in A. (Ai–Di) DAPI fluorescence images correspond to (A–D) show sperm nuclei location at varying time intervals, as indicated above each image. Sperm nuclei were stained by DAPI before fusion. (E) Bright field image of a fertilized egg cell kept in medium containing cytochalasin B for 2 hours. (Ei) Bright field and DAPI fluorescence image of the same cell in E. The bright white dot indicated the sperm nucleus, which remained at the entry site. Scale bar = 50 μm.